sch442416  (Cayman Chemical)

Journal: European Journal of Medical Research

Article Title: A 2A R regulate inflammation through PKA/NF-κB signaling pathways in intervertebral disc degeneration

doi: 10.1186/s40001-024-02028-7

Figure Lengend Snippet: Schedule of experiment and scoring. A Schematic diagram of the experimental procedure. B Survival curve. C HE staining and Safranin O-fast green staining in each group. D Histological score changes in the L5-6 intervertebral disk. Data represented mean ± SD (n = 4). E BBB scores. Data represented mean ± SD.* P < 0.05 versus S group, † P < 0.05 versus M group, ‡ P < 0.05 versus CGS-21680 group. S, sham operation group; M, IL-1β-induced IDD model group; CGS-21680, A 2A R agonist CGS-21680 group; SCH442416, A 2A R antagonist SCH442416 group

Article Snippet: Then, rats in CGS group received 50 μl (0.1 mg/kg, HY-13201A, Med Chem Express, Beijing, China) CGS-21680 injection [ ], and the rats in SCH group also received 50 μl (0.35 mg/kg, HY-103169, Med Chem Express, Beijing, China) SCH442416 injection [ ].

Techniques: Staining

Journal: European Journal of Medical Research

Article Title: A 2A R regulate inflammation through PKA/NF-κB signaling pathways in intervertebral disc degeneration

doi: 10.1186/s40001-024-02028-7

Figure Lengend Snippet: A 2A R expression in rat IDD model. A Immunofluorescence staining images of A 2A R of NP cells and the drawing of partial enlargement in box (positive expression indicated with the yellow arrows). B RT-PCR and C Western blot were used to detect the expression level of A 2A R in four groups. Data represented mean ± SD. Scale bars = 50 μm. * P < 0.05 versus S group, † P < 0.05 versus M group, ‡ P < 0.05 versus CGS-21680 group. S, sham operation group; M, IL-1β-induced IDD model group; CGS-21680, A 2A R agonist CGS-21680 group; SCH442416, A 2A R antagonist SCH442416 group

Article Snippet: Then, rats in CGS group received 50 μl (0.1 mg/kg, HY-13201A, Med Chem Express, Beijing, China) CGS-21680 injection [ ], and the rats in SCH group also received 50 μl (0.35 mg/kg, HY-103169, Med Chem Express, Beijing, China) SCH442416 injection [ ].

Techniques: Expressing, Immunofluorescence, Staining, Reverse Transcription Polymerase Chain Reaction, Western Blot

Journal: European Journal of Medical Research

Article Title: A 2A R regulate inflammation through PKA/NF-κB signaling pathways in intervertebral disc degeneration

doi: 10.1186/s40001-024-02028-7

Figure Lengend Snippet: Effects of A 2A R on the PKA/NF-κB pathway. A , B cAMP level was detected in the plasma and intervertebral disc tissues by ELISA. C RT-PCR results of PKA mRNA. D Images of PKA in each group with immunofluorescence staining in four groups and the drawing of partial enlargement in box (positive expression indicated with the yellow arrows). E – I Western-blot results of PKA, CREB and NF-κB in four groups. ( H , J , K ) RT-PCR results of CREB, NF-κB and NLRP3 mRNA. Scale bars = 50 μm. Data represented mean ± SD. Scale bars = 200 μm.* P < 0.05 versus S group, † P < 0.05 versus M group, ‡ P < 0.05 versus CGS-21680 group. S, sham operation group; M, IL-1β-induced IDD model group; CGS-21680, A 2A R agonist CGS-21680 group; SCH442416, A 2A R antagonist SCH442416 group

Article Snippet: Then, rats in CGS group received 50 μl (0.1 mg/kg, HY-13201A, Med Chem Express, Beijing, China) CGS-21680 injection [ ], and the rats in SCH group also received 50 μl (0.35 mg/kg, HY-103169, Med Chem Express, Beijing, China) SCH442416 injection [ ].

Techniques: Enzyme-linked Immunosorbent Assay, Reverse Transcription Polymerase Chain Reaction, Immunofluorescence, Staining, Expressing, Western Blot

Journal: European Journal of Medical Research

Article Title: A 2A R regulate inflammation through PKA/NF-κB signaling pathways in intervertebral disc degeneration

doi: 10.1186/s40001-024-02028-7

Figure Lengend Snippet: Effects of A 2A R on the inflammatory cytokines. A , B TNF-α and D , E IL-6 expression in the plasma and intervertebral disc tissues by ELISA. C , F RT-PCR results of TNF-α and IL-6 mRNA level. G – I Western-blot results of TNF-α and IL-6 protein in four groups. Data represented mean ± SD. Scale. * P < 0.05 versus S group, † P < 0.05 versus M group, ‡ P < 0.05 versus CGS-21680 group. S, sham operation group; M, IL-1β-induced IDD model group; CGS-21680, A 2A R agonist CGS-21680 group; SCH442416, A 2A R antagonist SCH442416 group

Article Snippet: Then, rats in CGS group received 50 μl (0.1 mg/kg, HY-13201A, Med Chem Express, Beijing, China) CGS-21680 injection [ ], and the rats in SCH group also received 50 μl (0.35 mg/kg, HY-103169, Med Chem Express, Beijing, China) SCH442416 injection [ ].

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Reverse Transcription Polymerase Chain Reaction, Western Blot

Journal: European Journal of Medical Research

Article Title: A 2A R regulate inflammation through PKA/NF-κB signaling pathways in intervertebral disc degeneration

doi: 10.1186/s40001-024-02028-7

Figure Lengend Snippet: Effect of A 2A R up-regulation on the expression of matrix metalloproteinase and type II collagen. A images of MMP3 in each group with immunofluorescence staining and and the drawing of partial enlargement in box (positive expression indicated with the yellow arrows) (Scale bars = 50 μm). B MMP3 and type II collagen levels were detected by western-blot. C , D Immunohistochemistry results of type II collagen in each group (Scale bars = 50 μm). Data represented mean ± SD.* P < 0.05 versus S group, † P < 0.05 versus M group, ‡ P < 0.05 versus CGS-21680 group. S, sham operation group; M, IL-1β-induced IDD model group; CGS-21680, A 2A R agonist CGS-21680 group; SCH442416, A 2A R antagonist SCH442416 group

Article Snippet: Then, rats in CGS group received 50 μl (0.1 mg/kg, HY-13201A, Med Chem Express, Beijing, China) CGS-21680 injection [ ], and the rats in SCH group also received 50 μl (0.35 mg/kg, HY-103169, Med Chem Express, Beijing, China) SCH442416 injection [ ].

Techniques: Expressing, Immunofluorescence, Staining, Western Blot, Immunohistochemistry